LysMCre-driven deletion of p15Ink4b in BM-DCs impairs their maturation. (A) Efficiency of LysMcre-driven deletion of p15Ink4b in sorted, LPS-treated BM-DCs derived from p15Ink4b^wt/wt-LysMcre and p15Ink4b^fl/fl-LysMcre mice. DNA was analyzed by qPCR for the p15Ink4b exon2 copy number, with p15Ink4b exon 1 used as an internal control. (B) Western blot of LPS-treated BM-DCs probed with anti-p15Ink4b Ab. For a loading control, the same blot was stripped and reprobed with anti-actin Ab. (C) LPS-treated BM-DCs from p15Ink4b^wt/wt-LysMcre (wt/wt; left panel) and p15Ink4b^fl/fl-LysMcre mice (fl/fl; right panel) were analyzed by FACS for the expression of MHC II, CD80, and CD86 molecules. Numbers in histograms indicate percentages of positive staining from 3 independent experiments (n = 7 for each genotype). (D) Bar graphs depicting the relative mean fluorescence intensity values of MHC II, CD80, and CD86 expression on gated positive populations of BM-DCs. Data are shown as means ± SD. *P < .05; n.s. indicates not significant.