Figure 3
![Figure 3. Depletion of HSCs in the BM of RbΔ/ΔE2f8Δ/Δ mice. (A-B) The absolute numbers of phenotypic stem cells (Lin−c-kit+Sca-1+ [LKS+]), progenitors (Lin−c-kit+Sca-1− [LKS−]), and long-term stem cells (Flk2−Lin−c-kit+Sca-1+) in the BM (A) and spleens (B) were estimated by flow cytometric analysis. Values above each bar in panel B represent the fold increases compared with control mice. (C-F) Photographs (C-D) and numbers (E-F) of CFU-S colonies from mice with the indicated genotypes. (G-H) Numbers of CFU-GEMM, CFU-GM, and BFU-E in the spleens (G) and BM (H) of mice with the indicated genotypes.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/119/19/10.1182_blood-2011-10-388231/4/zh89991290160003.jpeg?Expires=1724242411&Signature=F0eyqWcc23Rymz~-2u1i~PcS4~miXbz1LpJysQkoWrxxB-BFXEcy7C3SVEgLu~QaeWlm0E~y1oJJV084Gx7TZUv6MyZ-2TQzg7DBkN9VAzLKhUytIeG18xW-o9QlYgUMK7q2wGJhfVkD3KlgiTzYPm4Rz8fTQo0y2WsOKOSbT8WUNibyZUGHScgTrlEAMCVtK79vhJBenqTNeRGW5oEoLNO4cInMMrMK-QAxMFykAaOZQBOG9ALB0WdDDQHeaqlE9NM2J2aJPpfUmGuWZs7280SgFZNKAjYaIDCYVF927uqWhHHaR02S4Ncum19p21~wY2KAR4UwYdlImJusnCTz9g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Depletion of HSCs in the BM of RbΔ/ΔE2f8Δ/Δ mice. (A-B) The absolute numbers of phenotypic stem cells (Lin−c-kit+Sca-1+ [LKS+]), progenitors (Lin−c-kit+Sca-1− [LKS−]), and long-term stem cells (Flk2−Lin−c-kit+Sca-1+) in the BM (A) and spleens (B) were estimated by flow cytometric analysis. Values above each bar in panel B represent the fold increases compared with control mice. (C-F) Photographs (C-D) and numbers (E-F) of CFU-S colonies from mice with the indicated genotypes. (G-H) Numbers of CFU-GEMM, CFU-GM, and BFU-E in the spleens (G) and BM (H) of mice with the indicated genotypes.
