Biochemical alterations in the mitochondria in BCR-ABL1 leukemia cells. (A) BCR-ABL1-32Dcl3 cells (gray bars) and 32Dcl3 cells (black bars), and CD34⁺, CD34⁺CD38⁻ and CD34⁺CTV^max cells from CML-CP patients (gray bars) and healthy donors (black bars) were stained with JC-1 and the red/green fluorescence ratio is shown. (B) 32Dcl3 cells (black bars) and BCR-ABL1-32Dcl3 cells (gray bars) were used to measure electron transfer activity between MRC complexes I-III (left panel) and II-III (right panel). (C-D) Mitochondrial ·O₂⁻ was measured in 32Dcl3 cells (black bars) and BCR-ABL1-32Dcl3 cells (gray bars) by MSR (C) and mt-cpYFP fluorescence (D). (E-F) ROS were measured by DCFDA in CML-CP CD34⁺CD38⁻ and CD34⁺CD38⁺ subpopulations (E) and in CD34⁺CTV^max quiescent and CD34⁺CTV^low proliferative cells incubated (white bars) or not (gray bars) with mitochondria-targeted ubiquinone (F). Results represent mean values of a minimum of 3 measurements/group ± SD. *P < .05 compared with normal/parental (A-D) and untreated (E-F) cells.