Figure 2
Biochemical alterations in the mitochondria in BCR-ABL1 leukemia cells. (A) BCR-ABL1-32Dcl3 cells (gray bars) and 32Dcl3 cells (black bars), and CD34+, CD34+CD38− and CD34+CTVmax cells from CML-CP patients (gray bars) and healthy donors (black bars) were stained with JC-1 and the red/green fluorescence ratio is shown. (B) 32Dcl3 cells (black bars) and BCR-ABL1-32Dcl3 cells (gray bars) were used to measure electron transfer activity between MRC complexes I-III (left panel) and II-III (right panel). (C-D) Mitochondrial ·O2− was measured in 32Dcl3 cells (black bars) and BCR-ABL1-32Dcl3 cells (gray bars) by MSR (C) and mt-cpYFP fluorescence (D). (E-F) ROS were measured by DCFDA in CML-CP CD34+CD38− and CD34+CD38+ subpopulations (E) and in CD34+CTVmax quiescent and CD34+CTVlow proliferative cells incubated (white bars) or not (gray bars) with mitochondria-targeted ubiquinone (F). Results represent mean values of a minimum of 3 measurements/group ± SD. *P < .05 compared with normal/parental (A-D) and untreated (E-F) cells.

Biochemical alterations in the mitochondria in BCR-ABL1 leukemia cells. (A) BCR-ABL1-32Dcl3 cells (gray bars) and 32Dcl3 cells (black bars), and CD34+, CD34+CD38 and CD34+CTVmax cells from CML-CP patients (gray bars) and healthy donors (black bars) were stained with JC-1 and the red/green fluorescence ratio is shown. (B) 32Dcl3 cells (black bars) and BCR-ABL1-32Dcl3 cells (gray bars) were used to measure electron transfer activity between MRC complexes I-III (left panel) and II-III (right panel). (C-D) Mitochondrial ·O2 was measured in 32Dcl3 cells (black bars) and BCR-ABL1-32Dcl3 cells (gray bars) by MSR (C) and mt-cpYFP fluorescence (D). (E-F) ROS were measured by DCFDA in CML-CP CD34+CD38 and CD34+CD38+ subpopulations (E) and in CD34+CTVmax quiescent and CD34+CTVlow proliferative cells incubated (white bars) or not (gray bars) with mitochondria-targeted ubiquinone (F). Results represent mean values of a minimum of 3 measurements/group ± SD. *P < .05 compared with normal/parental (A-D) and untreated (E-F) cells.

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