Mutations found with TA cloning. (A) TA cloning found a novel, heterozygous in-frame insertion of 2 amino acids in the region of the previously described recurrent mutation in U2AF1. (B) In addition, a single occurrence of a somatic homozygous SRSF2 P95H mutation was seen as well as a novel missense SRSF2 P96S mutation adjacent to the previously described recurrent heterozygous point mutation at codon P95. (C) A novel, heterozygous 24 nucleotide in-frame deletion in SRSF2 was also found. (D) Multiple previously undescribed mutations were also found in ZRSR2, including several nonsense mutations, N-terminal insertions/deletions that resulted in premature stop codons, as well as in-frame insertions/deletions in the Arginine-serine rich domain.