Inhibition of HK3 by shRNA impairs neutrophil differentiation of APL cells. NB4 cells were stably transduced with nontargeting shRNA (SHC002) or shRNAs targeting HK3 (shHK3_560, shHK3_1420, shHK3_1748) and differentiated with 1μM ATRA for 6 days. HK3 (A) and CEBPE (B) mRNA levels were measured by real-time quantitative RT-PCR and are given as n-fold changes compared with untreated SHCOO2 cells and normalized to the housekeeping gene HMBS. HK3 protein expression was measured by Western blotting. GAPDH expression was used as loading control. (C-D) CD11b flow cytometric analysis of NB4 control (SHCOO2) and HK3 knockdown cells.***P < .0005; **P < .005