Transcriptional regulation of HK3 by PU.1 in NB4 and HT93 APL cell lines. NB4 and HT93 cells were stably transduced with nontargeting shRNA (SHC002) or shRNAs targeting PU.1 (shPU.1_256, shPU.1_928) and differentiated with 1μM ATRA for 6 days. HK3 and PU.1 mRNA expression as well as protein expression were measured in NB4 (A,C) or HT93 (B,D) cells by real-time quantitative RT-PCR and Western blotting. CEBPE mRNA expression was measured in NB4 (E) and HT93 (F) cells by real-time quantitative RT-PCR. CD11b flow cytometric analysis of NB4 (G) and HT93 (H) cells. ***P < .0001; **P < .01; *P < .05 (all P values Mann-Whitney U tests).