Figure 2
Recipients from miR-155–deficient splenocytes do not develop severe aGVHD and have increased survival. (A) Schematic presentation showing the different donor cells used in lethally irradiated F1 mice. (B) Clinical scores from the different recipient mice groups after transplantation. (C) Survival rate of lethally irradiated mice receiving BM alone, BM + miR-155−/− spleen, BM + miR-155+/+ spleen, or XRT alone. Data represent 2 independent experiments. (D) Histopathologic GVHD scores of recipient tissues. A second cohort of mice was used for histopathologic analysis. Fifteen days after transplantation (except for XRT group), mice from the indicated groups were killed and sections of the large bowel and liver were stained with H&E. Data are pooled from 2 independent experiments, representing approximately 6 or 7 animals per group, the XRT group that had 4 mice. There was no significant difference in the liver GVHD scores between the BM alone and BM + miR-155−/− spleen recipients (P = .07). The GVHD scores in the gut were higher in the BM + miR-155−/− spleen recipients versus the BM alone (P = .04). (E) Histopathologic evaluation of representative liver and large-bowel samples collected from the different recipient mice groups at day 15 after transplantation. The arrows in the large-bowel slide from the B6 BM + miR-155+/+ spleen F1 recipients showed lymphoplasmacytic infiltration in the lamina propia with frequent apoptosis The arrows in the liver slide from the same F1 recipients showed abundant lymphocytes infiltrating around the portal vein. No significant pathology is shown in BM alone and miR-155−/− groups. (F) Clinical GVHD scores after transplantation from lethally irradiated B10.BR recipients transplanted with BM alone, BM + miR-155−/−, or BM + miR-155+/+ spleen. (G) Survival rate of the different recipient B10.BR mice groups. (H) TNF-α levels measured in the serum of lethally irradiated F1 recipients of BM alone, BM + miR-155−/− spleen, and BM + miR-155+/+ spleen, 15 days after transplantation. Samples were assayed in triplicate and results are shown as the means from 4 biologic samples within a group.

Recipients from miR-155–deficient splenocytes do not develop severe aGVHD and have increased survival. (A) Schematic presentation showing the different donor cells used in lethally irradiated F1 mice. (B) Clinical scores from the different recipient mice groups after transplantation. (C) Survival rate of lethally irradiated mice receiving BM alone, BM + miR-155−/− spleen, BM + miR-155+/+ spleen, or XRT alone. Data represent 2 independent experiments. (D) Histopathologic GVHD scores of recipient tissues. A second cohort of mice was used for histopathologic analysis. Fifteen days after transplantation (except for XRT group), mice from the indicated groups were killed and sections of the large bowel and liver were stained with H&E. Data are pooled from 2 independent experiments, representing approximately 6 or 7 animals per group, the XRT group that had 4 mice. There was no significant difference in the liver GVHD scores between the BM alone and BM + miR-155−/− spleen recipients (P = .07). The GVHD scores in the gut were higher in the BM + miR-155−/− spleen recipients versus the BM alone (P = .04). (E) Histopathologic evaluation of representative liver and large-bowel samples collected from the different recipient mice groups at day 15 after transplantation. The arrows in the large-bowel slide from the B6 BM + miR-155+/+ spleen F1 recipients showed lymphoplasmacytic infiltration in the lamina propia with frequent apoptosis The arrows in the liver slide from the same F1 recipients showed abundant lymphocytes infiltrating around the portal vein. No significant pathology is shown in BM alone and miR-155−/− groups. (F) Clinical GVHD scores after transplantation from lethally irradiated B10.BR recipients transplanted with BM alone, BM + miR-155−/−, or BM + miR-155+/+ spleen. (G) Survival rate of the different recipient B10.BR mice groups. (H) TNF-α levels measured in the serum of lethally irradiated F1 recipients of BM alone, BM + miR-155−/− spleen, and BM + miR-155+/+ spleen, 15 days after transplantation. Samples were assayed in triplicate and results are shown as the means from 4 biologic samples within a group.

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