KAP1-mediated control of genes dysregulated in KAP1-deficient B cells. (A) Pten genomic locus with KAP1 and H3K9me3 ChIP/seq signal for Kap1 wild-type and KO B splenocytes. Chromosome and relative position (arrowhead), scale, UCSC-based transcripts (most abundant in black, least abundant in gray), CpG islands (suggestive of promoter regions), and position of Q-PCR primers used for validation in panels B, C, and D are depicted here as a1, a2, and b, respectively. Arrow indicates sense of transcription. (B-D) Chromatin from wild-type and KO B splenocytes was immunoprecipitated with KAP1-specific Abs (B), H3K9me3-specific Abs (C-D black bars), or H3Ac-specific Abs (D gray bars). Q-PCR in panels B, C, and D was performed with primers amplifying the region depicted in panel A and supplemental Figure 4 as a and b, respectively. Enrichment of immunoprecipitated samples is expressed as total input fraction (fold change over ctrl; C) normalized on the KO signal (fold change over KO; B,D). Average of the fold change in the control gene (GAPDH) was set at 1 (gray line). Averages plus SEM are shown (n = 4). *P < .05 compared with control gene signal by 1-tailed Mann-Whitney test. Also see supplemental Figure 5.