Transcriptome analyses of B-lymphoid KAP1-deficient splenocytes. (A) Number of genes dysregulated in T2-FP and FO Kap1-deleted cells compared with controls. Up arrow indicates the number of up-regulated genes; down arrow, number of down-regulated genes. (B) DAVID bioinformatics database analysis of dysregulated genes. Gene Ontology biological process classes of genes enriched in the dysregulated gene lists for T2-FP (top panel) and FO (bottom panel) KAP1-deficient cells. The fold enrichment and P values are shown. DDR indicates DNA-damage response; intrac, intracellular; metab, metabolic; proc., process; resp, response; biosynth, biosynthetic; rns, ribonucleoside; ns, nucleoside; and 3P, triphosphate. (C) The fold dysregulation of the depicted genes as assessed by array, Q-PCR on RNA extracted from C57/bl6-129sv CD19-Cre/Kap1flox–deficient and littermate wt/Kap1flox controls (Q-PCR mix) and Q-PCR on RNA extracted from C57/bl6 CD19-Cre/Kap1flox–deficient and CD19-Cre/Kap1wt controls (Q-PCR C57/bl6). Averages ± SD are shown (n = 3). Top panel shows the T2-FP population; bottom panel, FO population. p/a indicates present in Kap1-deleted and absent in control samples. Also see supplemental Figure 3 and supplemental Table 1.