Figure 2
Figure 2. Defective immune responses in B-lymphoid KAP1-deficient mice. (A) ELISA detecting total Ig was performed on CD19-Cre/Kap1flox (KAP1 KO) and littermate wt/Kap1flox control (ctrl) mouse sera either at steady-state (top left panel, naive) or 21 days after immunization with the indicated agents (middle and bottom panels). Right panel, CSR calculated as the ratio between the amount of indicated isotype and IgM in mice injected with NP-KLH or NP-LPS and alum. Average and SEM are shown (n ≥ 4). P values are by 1-tailed Mann-Whitney test. (B) Serum titers of neutralizing total Ig and IgG from CD19-Cre/Kap1flox (KAP1 KO) and littermate wt/Kap1flox control (ctrl) mice injected with rAD/VSV-G. Sera were analyzed at days 8, 12, 20, and 210. Mean and SEM are shown (n = 4). P values are by 2-way ANOVA test; only significant P values are depicted. Also see supplemental Figure 2.

Defective immune responses in B-lymphoid KAP1-deficient mice. (A) ELISA detecting total Ig was performed on CD19-Cre/Kap1flox (KAP1 KO) and littermate wt/Kap1flox control (ctrl) mouse sera either at steady-state (top left panel, naive) or 21 days after immunization with the indicated agents (middle and bottom panels). Right panel, CSR calculated as the ratio between the amount of indicated isotype and IgM in mice injected with NP-KLH or NP-LPS and alum. Average and SEM are shown (n ≥ 4). P values are by 1-tailed Mann-Whitney test. (B) Serum titers of neutralizing total Ig and IgG from CD19-Cre/Kap1flox (KAP1 KO) and littermate wt/Kap1flox control (ctrl) mice injected with rAD/VSV-G. Sera were analyzed at days 8, 12, 20, and 210. Mean and SEM are shown (n = 4). P values are by 2-way ANOVA test; only significant P values are depicted. Also see supplemental Figure 2.

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