Ablation of Jam-A results in prothrombotic phenotype. (A) Western blot analysis of proteins from Jam-A^wt/wt and Jam-A^gt/gt platelets. Human platelet lysate was used as a positive control. (B) Tail-bleeding time of Jam-A^wt/wt and Jam-A^gt/gt mice before genotyping (n = 32). Mean bleeding time is denoted by a horizontal line. (C) Carotid artery blood flow in Jam-A^wt/wt and Jam-A^gt/gt mice following exposure to 7.5% FeCl₃. Shown is the representative flow trace of Jam-A^wt/wt and Jam-A^gt/gt mice (n = 8). Duration of injury is denoted by no. 1 and the time to occlude is denoted by no. 2. (D) Quantitation of occlusion time from panel C.