Antibody response to full-length FVIII and to the C2 domain of FVIII. Mice were treated with human FVIII, intravenously (○ ●), or subcutaneously (▵ ▴), at weekly intervals for 8 consecutive weeks. One week after the last dose, plasma samples were collected and analyzed by ELISA for the presence of antibodies against FVIII (○ ▵) and against the C2 domain of human FVIII (● ▴). Presented are IgG antibody titers of individual mice.