Varying degrees of PML-RARA with different point mutations in response to As₂O₃ treatment. (A) Immunoblotting analyses for the expression of the long isoform of wild-type PML-RARA in HeLa cells treated with or without As₂O₃ for the indicated time course. The expression levels of endogenous Tubulin were detected as loading controls. (B) Immunoblotting analysis for the expression of Flag-tagged wild-type (PR-L-WT) and mutant PML-RARA in HeLa cells with or without As₂O₃ treatment (1 μM, 16 hours). (C) Immunoblotting analysis for the expression of Flag-tagged L217F and S220G mutants in HeLa cells treated with or without As₂O₃ (0.5 μM, 16 hours).