Example of 2D-DIGE analysis (using DeCyder software v6.5, GE Healthcare) of multiple isoforms of LSP1. (A) Focus of the 6 identified isoforms of LSP1 on 2D gel image. (B) Statistical analysis (3 UM-CLL vs 2M-CLL patient, #M3 patient excluded) of the 6 polypeptide spots identified as LSP1 with focus on 3 isoforms #1032, #1038, and #1043: #1032 acid isoform is significantly increased after anti-IgM stimulation in UM-CLL samples (*P < .0003), #1038 isoform presents no significant modification, and #1043 basic isoform is marginally decreased after anti-IgM stimulation. Such changes (increase of acid isoform and decrease of basic isoform) in 2D electrophoresis suggest a phosphorylation mechanism. (C) Focus of LSP1 isoforms on US/S UM-CLL samples 2D gel images (scanned using an Ethan DIGE Imager, GE Healthcare).