Example of 2D DIGE analysis of RAD23B protein expression. DIGE spots were analyzed with Decyder software Version 6.5 (GE Healthcare). (A) Statistical analysis of spot #929: significant decrease of RAD23B expression after BCR activation only in the UM-CLL subset. *P < .0001. (B) Decyder 3D representation of the RAD23B volume ratios difference between UM-CLL samples at baseline and after stimulation. (C) Focus of the RAD23B protein (spot #929) on US/S UM-CLL samples 2D gel images (scanned using an Ettan DIGE Imager, GE Healthcare).