Functional association of Gab2 with Lyn. (A) Overexpression of Gab2 WT and Gab2 DM were detected by Western blot in Ba/F3GR cells. Comparable protein levels were demonstrated by blotting for actin (bottom panel). (B) Overexpression of wild-type Gab2 induced prolonged dephosphorylation of pTyr507 but not prolonged auto-phosphorylation of Tyr396 of Lyn in response to G-CSF. Blots were stripped and reprobed with actin to demonstrate comparable protein loading. (C) Overexpression of Gab2 with mutations of Shp2 binding sites did not change phosphorylation status of Tyr507 or Tyr396 (as detected by anti-phospho Src Tyr416 antibody) of Lyn in response to G-CSF.