Gab2 associated with Shp2. (A) After transfection of HEK293 cells with Gab2 and Shp2, proteins immunoprecipitated with anti-Shp2 antibody were blotted for Gab2. (B) HEK293 cells were transfected with FLAG-tagged Gab2 wild-type (WT) and Y to F mutations at positions 614 or 643 or both (Gab2DM). After immunoprecipitation of Gab2, proteins were blotted for Shp2. The Western blot was stripped and probed for total Gab2 using the FLAG epitope antibody.