Mild versus severe hematopoietic phenotype in subsets of TG mice. (A-B) H&E staining of longitudinal sections of representative distal femora (A) from 4-week-old WT and TG littermates. Bars indicate 0.2 mm (low power) and 0.1 mm (high power). Quantification of LSK cells per limb (obtained by flushing 1 femur and 1 tibia per individual mouse limb, B) and frequency of spleen LSK cells (C). Spleen LSK-cell frequencies are also depicted by litter (D), demonstrating a mild (TG-A) and severe (TG-B) phenotype. Bars indicate mean and SEM. *P ≤ .05; **P ≤ .01.