Effects of panobinostat (LBH589) and everolimus (RAD001) combination on angiogenesis and glucose metabolism. (A) Cells were incubated with diluent control, LBH589 (0.02-0.1μM), RAD001 (0.1μM), or the combination of LBH589 and RAD001; and after 48 hours, cell lysates were prepared and resolved by SDS-PAGE, transferred to nitrocellulose membrane, and probed with anti–HIF-1α and anti-GLUT1 antibodies. (B) Cells were incubated with diluent control or LBH589 (0.02-0.1μM), RAD001 (0.1μM), or the combination of LBH589 and RAD001; and after 48 hours, supernatants were examined for VEGF levels using a multiplex assay. Each value represents a mean of 3 independent experiments ± SEM performed in triplicate.