Specificity of multipathogen-specific T-cell lines can be extended to CMV. (A) Precursor frequencies of T cells specific for the CMV pp65 peptide pool in PBMCs of 3 different donors assessed by IFN-γ ELISPOT assay. (B) Frequency of T cells specific for the CMV pp65 peptide pool after 14 days of expansion in single cultures. (C) Frequencies of T cells specific for the CMV pp65 peptide pool, the AdV hexon peptide pool, the EBV LMP2 peptide pool, and the C albicans MP65 peptide pool, and (D) in addition A fumigatus Crf1 peptide p41 after 14 days of expansion in multipathogen-specific cultures. Frequencies were determined by IFN-γ ELISPOT assay and are expressed as SFCs/2 × 105 cells (n = 2). The differences in the number of antigen-specific cells after expansion between single- and multipathogen-specific lines were not statistically significant.
