Virus-specific T cells in single- and multipathogen-specific cultures efficiently kill target cells. Specific lysis of negative control, partially matched, unpulsed LCLs, or partially matched LCLs pulsed with EBV LMP2 peptide pool by an EBV-specific T-cell line or CD8⁺ T cells isolated from an EBV-specific cell line (A) or a multipathogen-specific line (C). Specific lysis of negative control or partially matched LCLs pulsed with AdV hexon peptide pool by an AdV-specific T-cell line or CD8⁺ T cells isolated from an AdV-specific cell line (B) or a multipathogen-specific line (D). Specific lysis was determined with the ⁵¹Cr-release assay (representative experiment, n ≥ 2 for each of 2 donors).