Folding and dimeric stability of the WT and mutant CC2-LZ domains bearing D311N and D311G mutations. (A) Thermal denaturation curves of WT (●), D311N (□), and D311G (○), followed by CD at 222 nm, at a concentration of 8μM (0.1 mg/mL). (Inset) Far-UV CD spectra of WT, D311N, and D311G recorded at 1°C and at 8μM. (B) Secondary structure contents deduced from the deconvolution of the spectra at 1°C, melting temperatures (Tm), and cooperativities (n) calculated from the thermal denaturation profiles with a 2-state model.