miR-223 and NFI-A mRNA/protein levels in human hematopoietic cells and leukemias. (A) Quantitative RT-PCR of NFI-A mRNA level (□), Northern blot analysis of miR-223 expression, and immunodetection of NFI-A protein in CD34⁺ HSC/HPCs and CD34⁻ cells from PB or BM. U6 and β-tubulin were probed as RNA and protein loading controls, respectively. (B) Quantitative RT-PCR of miR-223 (■) and NFI-A (□) mRNA expression levels in leukemia patient samples classified by FAB.²⁴  ALL indicates acute lymphocytic leukemia; and NA, not available. Genetic features of leukemia blasts are indicated. Quantitative RT-PCR data are represented as mean ± SD of 3 independent evaluations.