The G-CSF receptor is expressed on peripheral sympathetic neurons and its stimulation reduces norepinephrine (NE) uptake. (A) Immunofluorescence staining for the G-CSF receptor (G-CSF-R; Cy3 top panel) or tyrosine hydroxylase (TH; Alexa 647, bottom panel) in sections of murine SCSG neurons. Images were acquired with a 40×/1.4 oil objective in a Zeiss AX10 microscope and a Coolsnap HQ2 camera (Fisher) at room temperature. Images were aquired using Slidebook 5.0 (3I). Purified rabbit immunoglobulin (TH) and isotype control (G-CSFR) are shown in insets. (B) G-CSF-R mRNA expression was assessed by RT-PCR in SCG neurons and whole BM. (C) SCSGs were cultured in medium containing PBS (n = 6), desipramine (DES; n = 6) at a concentration of 10μM, or 50 ng of G-CSF alone (n = 5) or in combination with anti–G-CSFR Ab (1 μg/mL; n = 3). *P < .05; **P < .005.