Reduced expression of CSE in transplant tolerance. (A) Tolerance is associated with a down-regulation of trans-sulfuration network genes. Gene regulation was assayed by microarray analysis at day 150 after transplantation in rats receiving an allogeneic kidney transplant and antidonor class II antibodies (empty bars) as the tolerizing regimen.21 Data are available on the Gene Expression Omnibus web-based data repository, under the GEO accession number GSE28474. APS Kinase indicates adenosine 5′-phosphosulfate kinase; CBS, cystathionine-β-synthase; CDO, cysteine dioxygenase; GCL, glutamate cysteine ligase; MPST, mercaptopyruvate sulfurtransferase; SO, sulfite oxidase; and TST, thiosulfate sulfurtransferase. Dara are mean ± SEM of 3 observations per group. **P < .01. ***P < .001. (B) Kidney transplants from rats receiving antidonor class II antibodies (Tolerant) and from syngeneic transplants (Syngeneic) were assayed for CSE expression by quantitative RT-PCR at day 100 after transplantation. Individual measurements are shown, and bars represent the mean value. *P < .05.
