Mice with FLT3/ITD-NHD13 mutations demonstrate altered Hox gene expression and stem cell expansion. (A) Quantitative RT-PCR analysis was performed on RNA extracted from the total bone marrow of 2-month-old WT/NHD13, FLT3/ITD, and FLT3/ITD-NHD13 C57Bl/6N littermates; Hox gene expression represents an average of 4 mice per genotype. (B) Meis1 expression of 2-month-old WT/NHD13, FLT3/ITD, and FLT3/ITD-NHD13 C57Bl/6N littermates. (C) Examples of sorted HSPC populations isolated from bone marrow are given for mice of each genotype. Bone marrow was stained for the cell surface markers characterizing the (D) KSL, (E) MPP, (F) ST-HSC, and (G) LT-HSC populations. Values in the graphs represent average percentages of these populations in the bone marrow from 3 independent experiments.