FLT3/ITD and NHD13 mutations cooperate to induce acute leukemia with high penetrance. (A) Schematic of FLT3/ITD knock-in construct and NHD13 transgene. An 18-bp sequence derived from a patient with AML was inserted into exon 14 of murine Flt3. (B) The Nup98-HoxD13 transgene contains the N-terminal GLFG repeats and the DNA binding homeodomain of HoxD13. (C) Examples of PCR genotyping results to detect the FLT3/ITD mutation and presence of the NHD13 transgenes from tail snip DNA. (D-E) Kaplan-Meier survival curves of WT, NHD13, FLT3/ITD, and FLT3/ITD-NHD13 mice (n = 20 in each group) for both the FVB/N and C57Bl/6N strains.