AML development by bone marrow transfer using Trib1 WT and R107L. (A) Kaplan-Meier survival curves are shown. The P value was calculated with the log-rank test. (B) Immunoblot analysis of Trib1 WT AML (Mac-1 56.2%, Gr-1 52.5%, CD34^lo, c-kit⁻, Sca-1⁻) and R107L AML (Mac-1 41.4%, Gr-1 25.2%, Cd34^lo, c-kit^lo, Sca-1⁻) derived from bone marrow of recipient mice (WT #T73 and R107L #T151 in supplemental Table 1). Enhancement of ERK phosphorylation is more significant in R107L. Relative values of ERK phosphorylation were calculated by densitometric analysis. (C) Immunoblot analysis for C/EBPα of the same AML samples as in panel B. Relative expression level of C/EBPα is quantitated (right).