CalDAG-GEFI deficiency protects from immunoglobulin-induced thrombosis in vivo. (A-B) Platelet counts in whole blood at baseline and 4 hours after infusion of anti-GPIX antibody. (C-D) Representative near-infrared images of lungs extracted from the respective mice 4 hours after anti-GPIX-Alexa750 injection obtained with a LICOR Odyssey scanner, software; LICOR Odyssey, Version 3.0. (E-F) Quantitative analysis of the integrated fluorescence density (Adobe Photoshop CS3 extended, Version 10.0.1 Adobe). Results shown are mean fluorescence intensity (a.u.) ± SEM; n = 3 to 5. WT (checkered bar), transgenic hFcR (black bar), hFcR pretreated with 75 mg/kg body weight clopidogrel (light gray bar), and hFcR/CDGI^−/− (dark gray bar) mice were injected with an Alexa750-labeled antibody against GPIX at a dosage of 0.5 μg/g (A,C,E) or 1 μg/g (B,D,F) body weight. ***P < .001. **P < .01. *P < .05. ns indicates not significant.