Surface expression of human FcγRIIA. (A) Representative histograms for platelets from a WT mouse (dashed black trace) and a mouse transgenic for the hFcR (black trace) stained with IV.3-Alexa488. (B) FcγRIIA expression levels on platelets from hFcR mice and mice transgenic for hFcR and deficient in CalDAG-GEFI (hFcR/CDGI^−/−). Only mice with a platelet mean fluorescence signal (IV.3-Alexa488) between 100 and 150 were used for further experiments.