IL-10 induces JAK2-pathway activation in DLBCL cells. (A) Serum-starved DHL2 and DHL6 cells were treated with 100 ng/mL of rIL-10 for various times as indicated and phosphorylation of JAK2 and JAK1 was assessed by Western blot. (B) Serum-starved DHL2, Ly3, and DHL6 cells were treated with rIL-10 for 5, 15, and 30 minutes and STAT3 tyrosine and serine phosphorylation was assessed by Western blot. (C) Serum-starved DHL2 and DHL6 cells were treated with 100 ng/mL of rIL-10 and phosphorylation of STAT1 and STAT5 were analyzed. (D) Serum-starved DHL2 cells were treated with 100 ng/mL of rIL-10 for various times and expression of c-myc and survivin were analyzed by Western blot. (E) Serum-starved DHL2 cells were treated with neutralizing IL-10 Ab, followed by IL-10, and JAK2 and STAT3 phosphorylation was analyzed.