Effects of HIF1α deficiency on normal HSCs. (A) RT-PCR analysis for the expression of HIF1α, VEGF, GLUT1, TGFα, and PGK1 in WT or HIF1α^−/− normal LSK cells and BCR-ABL–expressing LSK cells. (B) Gene set enrichment analysis of DNA microarray data displays gene expression profiling of HIF1α targets in WT or HIF1α^−/− normal LSK cells and BCR-ABL–expressing LSK cells. (C) FACS analysis of HSCs in BM cells from WT or HIF1α^−/− mice. The percentages of LSK cells (Lin⁻Sca-1⁺c-Kit⁺), LT-HSCs (Lin⁻Sca-1⁺c-Kit⁺CD34⁻CD135⁻), and ST-HSCs (Lin⁻Sca-1⁺c-Kit⁺CD34⁻CD135⁺) were similar in WT and HIF1α^−/− mice; however, HIF1α^−/− mice showed a higher percentage of MPP (Lin⁻Sca-1⁺c-Kit⁺CD34⁺CD135⁺) (n = 5). (D) Similar percentages of CD41⁻CD48⁻CD150⁺ Lin⁻Sca-1⁺c-Kit⁺ cells in BM cells of WT or HIF1α^−/− mice (n = 3).