Effects of HIF1α deletion on retroviral transduction efficiency and the homing ability of normal and BCR-ABL–transduced totalBMcells or LSK cells. (A) HIF1α deletion does not affect retroviral transduction efficiency. WT and HIF1α^−/− BM cells were transduced with empty vector or BCR-ABL-GFP retrovirus, and 2 days later, the percentages of GFP⁺ cells were determined by FACS. (B) FACS analysis showed the similar homing ability of WT and HIF1α^−/− BM cells. A total of 3 × 10⁶ BM cells from WT or HIF1α^−/− mice (CD45.2) were transplanted into lethally irradiated recipients (CD45.1). The donor-derived BM cells (CD45.2) were detected by FACS in 3 hours after BMT (n = 4). Mean values (± SD) are shown. NS indicates no significance. (C) HIF1α deletion does not cause a reduction of the homing ability of BCR-ABL–transduced BM cells. A total of 2 × 10⁶ leukemia cells (GFP⁺) from CML mice transplanted with BCR-ABL–transduced WT or HIF1α^−/− BM cells were transplanted into lethally irradiated recipients (CD45.1). Three hours later, donor-derived leukemia cells (GFP⁺) in the BM were detected by FACS (n = 4 for each group). Mean values (± SD) are shown; **P < .01. (D) HIF1α deletion does not cause a reduction of the homing ability of control or BCR-ABL–transduced stem cells. BM cells were transduced with GFP vector or BCR-ABL-GFP, and 2 × 10⁴ sorted normal LSK cells and BCR-ABL–expressing LSK cells (GFP⁺Lin⁻Sca-1⁺c-Kit⁺) were transplanted into lethally irradiated recipients (CD45.1). Three hours later, donor-derived leukemia cells (GFP⁺) in the BM were detected by FACS (n = 3 for each group). Mean values (± SD) are shown.