American Society of Hematology

Figure 3

$Figure 3. Blockade of the PD-1 pathway augments the fraction of HIV-specific IFN-γ+ CD4 T cells in both proliferating and nonproliferating subsets. (A-B) CFSE-labeled, CD8-depleted PBMCs were incubated with no antigen (A) or HIV Gag peptide (B) in the presence of PD-L1 blocking antibody or isotype control antibody. Intracellular cytokine staining was detected at 48, 72, 96, and 168 hours after stimulation. (C) The effect of PD-L1 blockade on IFN-γ+ CD4 T cells at 96 hours after stimulation in 3 representative subjects.$

Blockade of the PD-1 pathway augments the fraction of HIV-specific IFN-γ⁺ CD4 T cells in both proliferating and nonproliferating subsets. (A-B) CFSE-labeled, CD8-depleted PBMCs were incubated with no antigen (A) or HIV Gag peptide (B) in the presence of PD-L1 blocking antibody or isotype control antibody. Intracellular cytokine staining was detected at 48, 72, 96, and 168 hours after stimulation. (C) The effect of PD-L1 blockade on IFN-γ⁺ CD4 T cells at 96 hours after stimulation in 3 representative subjects.

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