RAD51(pY315) is detected in the nuclei of BCR-ABL1-positive leukemia cells. (A) RAD51(pY54), RAD51(pY315), and total RAD51 were detected by Western blotting with the use of phospho-specific and with anti-RAD51 antibodies, respectively, in the cytoplasmic and nuclear lysates from 32Dcl3 parental cells (32D) and BCR-ABL1–positive counterparts (B/A). The amount of protein lysates run in the gel was adjusted to detect similar levels of RAD51. (B) Flag-RAD51(WT) and Flag-RAD51(Y315F) were expressed in B/A cells, and Flag-tagged RAD51 proteins were detected in total, nuclear, and cytoplasmic cell lysates by Western analysis using anti-Flag antibody (Flag-RAD51 bands are marked by arrow). Lamin and tubulin served as the lysates purity controls.