MiR-17/20/93/106 expression levels during mouse and human neutrophil differentiation. (A) 32D-CSF3R cells were switched from IL-3 to CSF3-containing medium on day t = 0. Micrographs show the morphology of 32D-CSF3R cells on indicated time points of CSF3 treatment. Bars indicate 10 μm. Total RNA was isolated at indicated time points. Expression of indicated mature miRNAs was measured in triplicate with quantitative RT-PCR. The miRNA expressions relative to SnoRNA-234 were measured from immature (steady state, IL-3) samples and set to 1. The average fold expression (n = 3) at indicated time points relative to the IL-3 condition are shown. Error bars represent SD. Significance was calculated by comparing the samples of steady state and the different time points of CSF3 treatment with the Mann-Whitney test (asymptotic significance [2-tailed]). *P < .05. (B) Indicated myeloid cell fractions from human cord blood were stained with cell type specific antibodies and isolated using FACS sort. Micrographs show morphology of sorted cell fractions. Bars indicate 10 μm. MiRNA levels and significance were measured and calculated as described in panel A.