In vivo binding of GATA1 to the membrane protein genes. The in vivo association of GATA1 with Slc4a1 (A) and Aqp1 (B) genes was evaluated with ChIP assay in fetal livers of WT (top panel) and V205GR-A (bottom panel) mice. DNA fragments immunoprecipitated with GATA1-N6 antibody were diluted 1, 2, and 3 times, and rendered for 35 cycles of amplification. Samples of the input DNA were used in the PCR reactions to confirm that equal amounts of DNA were present in the samples. A control immunoprecipitation reaction with control IgG was also included.