AZD6244 induces decrease in cell-cycle regulatory and antiapoptotic proteins. (A) Cells were incubated with 200nM and 300nM AZD6244 for 18 hours. Protein levels were measured in whole cell lysates by immunoblotting using indicated antibodies. (B) Down-regulation of MCL1, BCL2 and c-MYC in the whole cell lysate of PBMC afterAZD6244 exposure for 18 hours. (C) Induction of p27 by AZD6244 in all the cell lines afterincubation with 200nM AZD6244 for 18 hours. (D) SUDHL6 and OCI-LY3 cells were incubated with AdM4 Myc reporter or mutant Myc reporter adenovirus for 4 hours. After 24-hour transduction, cells were incubated with 200nM AZD6244 in SUDHL6 and 300nM in OCI-LY3 cells for 24 hours followed by luciferase assay.