Functional implications in leukemogenesis of mutated RUNX1 proteins in vivo. (A) WB analysis of BCR-ABL and RUNX1 mutant expression in BM cells of mouse transplanted with stem cells coinfected by BCR-ABL and RUNX1 mutant retroviruses. (B) Quantification of the myeloblast/promyelocyte cells in BM. (C) Quantification of CD117⁺ cells in the BM. (D) Morphological analysis (left) and immunophenotype analysis (right) of hematopoietic cells from representative diseased mice. BM cytocentrifugation is shown by Wright staining. (E) Kaplan-Meier survival curves of mice transplanted with BM cells transduced with the indicated retroviral constructs. (F) Survival curves of secondary recipient mice transplanted with leukemic cells from BCR-ABL, BCR-ABL plus H78Q, or BCR-ABL plus V91fs-ter94–transduced mice.