Figure 1
FL-MSCs display a specific gene expression profile. (A) Hierarchical clustering of resting untreated HD-MSCs (n = 6) and FL-MSCs (n = 10). Analysis was performed on the 9967 PS showing the greatest variation coefficient between samples. The relative level of gene expression is depicted according to the shown color scale. (B) Schematic representation of the statistical analysis used to highlight the FL-MSC signature defined as the intersection of the 2 genelists generated by SAM analysis (FC > 2 and false discovery rate < 5%) and Mann-Whitney rank test (FC > 2 or < 0.5 and P < .05). (C) Validation by RQ-PCR of the overexpression of CHST2, CLU, DUSP6, TLR4, TNFRSF10A, and CCL2 in FL-MSCs compared with HD-MSCs. Each sample was normalized to GAPDH, and the arbitrary value of 1 was assigned to the median expression of HD-MSCs. *P < .05; ***P < .001.

FL-MSCs display a specific gene expression profile. (A) Hierarchical clustering of resting untreated HD-MSCs (n = 6) and FL-MSCs (n = 10). Analysis was performed on the 9967 PS showing the greatest variation coefficient between samples. The relative level of gene expression is depicted according to the shown color scale. (B) Schematic representation of the statistical analysis used to highlight the FL-MSC signature defined as the intersection of the 2 genelists generated by SAM analysis (FC > 2 and false discovery rate < 5%) and Mann-Whitney rank test (FC > 2 or < 0.5 and P < .05). (C) Validation by RQ-PCR of the overexpression of CHST2, CLU, DUSP6, TLR4, TNFRSF10A, and CCL2 in FL-MSCs compared with HD-MSCs. Each sample was normalized to GAPDH, and the arbitrary value of 1 was assigned to the median expression of HD-MSCs. *P < .05; ***P < .001.

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