American Society of Hematology

Figure 1

$Figure 1. DAF expression on ovalbumin-specific TCR-transgenic T cells does not affect T-cell response to specific antigen stimulation. (A) FACS-staining analysis confirmed that both CD4+ and CD8+ T cells from Daf+/+ but not Daf−/− TCR transgenic mice expressed high levels of DAF on their surface. (B,C) Purified Daf+/+ or Daf−/− OT-II CD4+ (right panels) or OT-I CD8+ (left panels) T cells were cocultured with irradiated unfractionated splenocytes of C57BL/6 WT mice for 3 days in the presence of different concentrations of peptide OVA323-339 or OVA257-264, respectively. T-cell proliferation (B) was measured by 3H-TdR incorporation and IFN-γ production (C) was measured by ELISA. Data are representatives of 3 independent experiments. NS indicates not significant. P > .05.$

DAF expression on ovalbumin-specific TCR-transgenic T cells does not affect T-cell response to specific antigen stimulation. (A) FACS-staining analysis confirmed that both CD4⁺ and CD8⁺ T cells from Daf^+/+ but not Daf^−/− TCR transgenic mice expressed high levels of DAF on their surface. (B,C) Purified Daf^+/+ or Daf^−/− OT-II CD4⁺ (right panels) or OT-I CD8⁺ (left panels) T cells were cocultured with irradiated unfractionated splenocytes of C57BL/6 WT mice for 3 days in the presence of different concentrations of peptide OVA_323-339 or OVA_257-264, respectively. T-cell proliferation (B) was measured by ³H-TdR incorporation and IFN-γ production (C) was measured by ELISA. Data are representatives of 3 independent experiments. NS indicates not significant. P > .05.

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