PRDX2 expression inhibits c-Myc-induced colony formation in primary murine BM. (A) Design of the retroviral constructs used for transduction of murine BM cells. pMSCV vector backbone with IRES-GFP. c-Myc was cloned in front of IRES-GFP (MIG). PRDX2 was cloned together with second IRES in front of c-Myc (PIMIG). (B) Western blot analyses confirmed the expression of c-Myc and PRDX2 in 32D cells transduced with the indicated retroviral constructs. Actin was used as loading control. (C) PRDX2 overexpression in murine BM cells inhibited the colony growth that was induced because of Myc overexpression. The results shown are the mean ± SD of 3 independent experiments (GFP vs c-Myc, P = .027; c-Myc vs PRDX2, P = .012, t test). (D) Serial replating indicated the decrease of replicative potential of c-Myc–transduced cells with simultaneous expression of PRDX2. The results shown are the mean ± SD of 2 independent experiments (*P < .05, calculated by t test). (E) Photographs taken on day 8 of colony assays showing enhanced colony formation in c-Myc–expressing BM cells. PRDX2 expression together with c-Myc reduced the number of colonies in total. The colonies in c-Myc–PRDX2 appeared as more differentiated colonies from granulocytic/monocytic lineage with more scattered structure, whereas in c-Myc alone, they appeared as colonies from more immature/myeloid progenitor cells.