Patterns of CpG methylation of the human androgen receptor gene HUMARA by bisulfite sequencing. (A top) Diagram of the 5′ region of the human androgen receptor gene showing the locations of core promoter region, transcription start site (+1), translation start site (ATG), and methylation-sensitive enzyme sites for HpaII. Specific CpG dinucleotides in exon1 region are highlighted in the sequence of PCR product. (down) The sequencing results of the PCR products of the exon 1 HUMARA gene in native selected granulocytes. Total 20-29 clones were analyzed from healthy controls YC25, YC17, YC3, and YC18, respectively. The white dots represent the unmethylated CpGs, whereas black dots represent the methylated CpGs. Short allele (A) and long allele (B) were determined according to the length of the AR gene exon 1 microsatellite. (B) Patterns of CpG methylation of the human androgen receptor gene HUMARA by bisulfite sequencing in individual BFU-Es of subject YC17. The sequencing results of the PCR products of the exon 1 HUMARA gene. Total 6-10 clones were analyzed for BFU-E 1, BFU-E 2, BFU-E 3, BFU-E 4, BFU-E 5, and BFU-E 6, respectively. The white dots represent the unmethylated CpGs, whereas the black dots represent the methylated CpGs. Short allele (A) and long allele (B) were determined according to the length of the AR gene exon 1 microsatellite.