DAPK2- and PIM1-derived nonameric peptides elicit T-cell reactivity in post-HSCT PBMCs from patient A. Fresh post-HSCT PBMCs were stimulated ex vivo with 10μM concentrations of individual peptides in the presence of 10 μg/mL of IL-7 for 7 days, then tested by ELISpot with peptide-pulsed autologous matured dendritic cells (PBMC:APC ratio = 1:1). (A) T cells from patient A 2.5 years after HSCT show reactivity to the DAPK2-derived peptide D1, but not to other DAPK2-derived peptides. (B) T cells from patient A 2.75 years after HSCT are strongly reactive to the PIM1-derived peptide P4 and weakly reactive to P1, P2, P5, and P7.