Figure 7
siRNA targeting Axl or CDCP-1 decrease nilotinib resistance. K-rn cells were transfected with 200nM of siRNA corresponding to a negative control siRNA (siRNA Neg) or targeted siRNA; siRNA Axl (A) or siRNA-CDCP-1 (B). Cells were seeded at 2 × 105/mL in RF-10 for 24 hours after incubated in the presence of 20nM nilotinib. Aliquots of the culture were harvested at day 2 for measurement of cell death by triplicate trypan blue exclusion counts and protein expression analysis. Results are from one experiment representative of 2.
