Inhibition of Syk expression altered phosphorylation and interaction of Lyn, Axl and CDCP-1. Lyn (A), Axl (B) and CDCP-1 (C) were immunoprecipitated from K-rn and K-rn sh-Syk cells. After transfer, membranes were probed for tyrosine phosphorylated proteins using a mix of 4G10 and pY-100 antibodies (top panels). After stripping, membranes were cut and reprobed as indicated. Results are from 1 experiment representative of at least 3.