Silencing Syk restores nilotinib sensitivity. K-rn cells were infected by lentivirus coding for a shRNA anti-Syk, grown for 10 days, selected for DsRed expression and purified by cell sorting. Syk silencing was confirmed by Western blotting (A) and apoptosis in response to nilotinib was measured on both K-rn and K-rn ShRNA Syk cells by cytometry using annexin V-FITC (B). Results are expressed as the percentage of annexin V positive cells.