Blocking Tim-3 decreases IFN-γ production in primary human NK cells and has a minimal effect on degranulation. (A-B) IL-12 (1 ng/mL) and IL-18 (10 ng/mL) activated PBMCs were coincubated with Raji eGFP and Raji Gal-9 target cell lines in the presence of a blocking Tim-3 mAb or isotype control for 5 hours at an E:T ratio of 10:1. (A) Intracellular IFN-γ production (*P < .05, n = 6) and (B) CD107a expression (n = 6) was determined by FACS analysis (error bars represent SEM).