Down-regulated expression of TNFα in LPS-activated BM cells transfected with Pre-miR-181c. (A) Representative flow cytometric analysis showing intracellular expression of TNFα in CD14⁺ cells in fresh BM and BM cells activated with PMA, LPS, or R848. (B) Representative analysis of intracellular TNFα in LPS-activated BM cells transfected with Pre-miR control or with Pre-miR-181c. Gray histogram corresponds to nonactivated cells; empty histograms correspond to LPS-activated cells transduced with the Pre-miR control (dotted line) and with Pre-miR-181c (continuous line). Data correspond to a BM sample from an HD.