Pre-miR-181c regulates the expression of TNF-α by interacting with its 3′UTR sequence. Shown is the normalized Renilla luciferase activity in RKO cells transfected with the different constructs. (A) RKO cells were transfected with the different siCHECK2 plasmids. (B) RKO cells were co-transfected with Pre-miR control (white bars) or Pre-miR-181c (black bars) together with the different siCHECK2 plasmids. (C) LCLs from HDs were transfected with siCHECK2-mutTNFα or siCHECK2-TNFα and Pre-miR-181c. (D) RKO cells were co-transfected with anti-miR control (white bars) or with an anti–miR-181c (black bars) together with the different siCHECK2 plasmids. In all cases, Renilla luciferase activity was normalized to firefly luciferase activity. Bars show mean values ± SE corresponding to 3-5 independent experiments.